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Image Search Results
Journal: Cells
Article Title: TRPV4 Inhibition and CRISPR-Cas9 Knockout Reduce Inflammation Induced by Hyperphysiological Stretching in Human Annulus Fibrosus Cells
doi: 10.3390/cells9071736
Figure Lengend Snippet: Representative Western blots from one donor ( A – C ) and densitometry analysis ( D – F ) of the phosphorylated and total ( A , D ) ERK 1/2; ( B , E ) p38; and ( C , F ) JNK immediately after no (white bar) or 15 min (grey bars) of cyclic stretching at 20% strain and 1 Hz in the absence or presence (hatched bars) of 20–500 nM of the TRPV4 antagonist GSK2193874. Non-stretched cells treated with IL1β were used as a positive control for the blots. One same blot of α-tubulin is shown three times as a loading control. n = 3 donors ( n = 2 for p-JNK); mean ± SD; * p < 0.05, ** p < 0.01.
Article Snippet: As a positive control, the cells were pre-treated for 15 min with 10 ng/mL
Techniques: Western Blot, Positive Control, Control